PBST (Phosphate Buffered Saline with Tween-20), sterilized by 0.22 μm filtration, DNase & RNase free, Protease-free, Pyrogen-free, is a phosphate buffered saline containing Tween-20. It can provide a relatively stable ionic environment and pH buffering capacity. It is a buffered saline solution frequently used in biology. It is mainly used in experiments such as immunohistochemistry, in situ hybridization and enzyme-linked immunosorbent, and for washing immunoblot membranes. Tween-20, as a detergent, can increase the elution capacity of the buffer.
Composition
| NaCl | 136 mM |
| KCl | 2.6 mM |
| Na2HPO4 | 8 mM |
| KH2PO4 | 2 mM |
| Tween-20 | 0.05 % (V/V) |
Product properties
| Appearance |
Colorless clear liquid |
| pH | 7.4 - 0.1 |
Storage & Shelf Life
Store at room temperature, valid for 3 years.
Ordering information
| Cat # | Description | Package |
| PBST-500 | PBST buffer (1×) | 500mL/Bottle, 10 Bottles/Case |
| PBST-500-1 | PBST buffer (1×) | 500mL/Bottle |
