Biologix® Western Transfer Buffer ( 10X ), PH8.3, 500mL/ Bottle, 10 Bottles/Case
£31.39
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Biologix® Western Transfer Buffer ( 10X ), PH8.3, 500mL/ Bottle, 10 Bottles/Case
Product Details
Western transfer buffers are used in Western blotting (WB) experiments to transfer proteins from gels to membranes, and then to semi-quantitative detection of proteins in complex samples based on antigen-antibody specific binding.
This product is a 10× concentrate and needs to be diluted as follows before use: Take 100 mL of 10× transfer buffer, add 200 mL of anhydrous methanol and mix well, then add distilled water or deionized water to make up to 1 L, mix well and it can be used.
Features
High quality: The buffers are filtered by 0.22 µm to ensure the highest quality.
Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation time while reducing the likelihood of buffer concentration errors.
DNase & RNase free, Protease-free, Pyrogen-free.
Composition
|
Tris |
250 mM |
|
Glycine |
1.92 M |
Product Properties
|
Appearance |
Colorless clear liquid |
|
pH |
8.3±0.1 (diluted to 1×) |
|
Sterile |
Non-sterile |
Storage & Shelf Life
Store at room temperature, valid for two years.
Ordering Information
| Cat # | Description | Package |
| WTB-510-1 |
Western Transfer Buffer (10×) |
500mL/ Bottle |
| WTB-510 | Western Transfer Buffer (10×) | 500mL/ Bottle, 10 Bottles/Case |
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