Biologix® Tris-glycine-SDS (TGS) Electrophoresis Buffer ( 1X ), pH8.3, 500mL/Bottle

Tris-glycine-SDS (TGS) electrophoresis buffer contains SDS and is commonly used as a buffer for denatured protein polyacrylamide gel electrophoresis. Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) is the electrophoresis of denatured proteins on polyacrylamide gels with the addition of denaturants such as SDS and ethanol.

SDS is an anionic surfactant. As a denaturant and solubilizing agent, it can break the hydrogen bonds within and between molecules, unfold the molecules, and destroy the secondary and tertiary structures of protein molecules. Strong reducing agents such as mercaptoethanol and dithiothreitol can break the disulfide bonds between cysteine residues. After adding reducing agents and SDS to the sample and gel, the molecules are depolymerized into polypeptide chains. The depolymerized amino acid side chains and SDS combine to form protein-SDS micelles. The negative charge carried by them greatly exceeds the original charge of the protein, thus eliminating the charge differences and structural differences between different molecules. The mobility of the protein mainly depends on its relative molecular mass.

Composition

Product Properties

Storage & Shelf Life

Store at room temperature, valid for 2 years.