TBE-510 TBE Buffer (10×), pH 8.3 890 mM Tris-borate, 20 mM EDTA 500 mL/Bottle, 10 Bottles/Case

can help standardize your electrophoresis runs, as they are made from purity-tested reagents through Biologix electrophoresis separation, ensuring reproducibility through quality control.

Features

High quality: The buffers are filtered by 0.22 µm to ensure the highest quality.

Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation

time while reducing the likelihood of buffer concentration errors.

DNase & RNase free, Protease-free, Pyrogen-free.

Selection Guide

● Nucleic acid electrophoresis

When high resolution is not necessary, both TAE and TBE buffers are suitable. For higher resolution requirements, using a

lower concentration gel is advantageous for improving the resolution of large molecular weight nucleic acids (>13kb), and TAE

is recommended. Conversely, higher concentration gels are beneficial for enhancing the resolution of small molecular weight

nucleic acids, and TBE is preferable in this case.

● Protein Electrophoresis

Neutral pH buffers for the Bis-Tris gel system help to improve sample integrity and gel stability, resulting in clearer, sharper

bands and higher resolution for low protein abundance or downstream applications requiring high protein integrity. MOPS

buffer is recommended for separating proteins of 15-260 kDa. As for the proteins of 3.5-160 kDa, MES buffer is recommended.

Electrophoresis on Tris-Glycine gel system, TGS (TG+0.1% SDS) buffer is recommended.

● Protein blotting

Transfer buffers are used in Western blotting (WB) experiments to transfer proteins from gels to membranes, and then to semi

quantitative detection of proteins in complex samples based on antigen-antibody specific binding.

Storage & Shelf Life

TAE, TBE, MOPS, MES: Store at 2-30℃ . Shelf life of 36 months.